Return to CAP Home

  Diagnostic Immunology

  This page and related links last reviewed on August 15, 2013

Resources Resources

Discussions

Articles

References

  • International Myeloma Working Group guidelines for serum-free light chain analysis in multiple myeloma and related disorders (PDF, 347 KB)
  • International Uniform Response Criteria for Multiple Myeloma (PDF)
  • NCCN guidelines on multiple myeloma (PDF, 300 KB)
  • NIH Consensus Development Conference on Celiac Disease
  • 2006 Bethesda International Consensus Recommendations on the Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications (The Cytometry Part B: Clinical Cytometry Supplement 72B:S5-S13, 2007)
  • 2006 Bethesda International Consensus Recommendations on the Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Optimal Reagents and Reporting for the Flow Cytometric Diagnosis of Hematopoietic Neoplasia (The Cytometry Part B: Clinical Cytometry Supplement 72B:S14-S22, 2007)
  • 2006 Bethesda International Consensus Recommendations on the Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Recommendations for Training and Education to Perform Clinical Flow Cytometry (The Cytometry Part B: Clinical Cytometry Supplement 72B:S23-S33, 2007)
  • The International Consensus Statement on Testing and Reporting of Antineutrophil Cytoplasmic Antibodies ([ANCA] Savige J, Gillis D, Benson E, et al. Am J Clin Path. 1999;111:507-513).
  • The Addendum to the International Consensus Statement on Testing and Reporting of ANCA: Quality Control Guidelines, Comments, and Recommendations for Testing in Other Autoimmune Diseases (Savige J, Dimech W, Fritzler M, et al. Am J Clin Path. 2003;120:312-318).
  • The International Consensus Statement on an update of the Classification Criteria for Definite Antiphospholipid Syndrome [(APS) Miyakis S, Lockshin MD, Atsumi T, et al. J Thromb Haemost. 2006;4:295-306)
  • Consensus Guidelines on Anti-Beta 2 Glycoprotein I Testing and Reporting (Wong RCW, Favaloro EJ, Adelstein S, et al. Pathology. 2008;40:58-63)
  • Update of the Guidelines for Lupus Anticoagulant Detection (Pengo V, Tripodi A, Reber G, et al. J Thromb Haemost. 2009;7:1737-1740).
  • Integrated Hematopathology: Morphology and FCI with IHC by Cherie Dunphy, MD (ASCP Press, 2010) offers a comprehensive flow cytometry textbook which covers the technical aspects of flow cytometry, phenotypic markers, and the advantages and disadvantages of flow cytometry and can be used as a quick diagnostic guide incorporating normal and abnormal phenotypic findings of peripheral blood, body fluids, bone marrow, and lymph nodes. Integration of flow cytometry immunophenotyping with morphology and molecular genetic findings provides a complete diagnostic picture.

CLSI References

  • Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; Approved Guideline – Second Edition (Clinical and Laboratory Standards Institute, document H43-A2)
  • Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline – Second Edition (Clinical and Laboratory Standards Institute, document H42-A2)
  • Evaluation Methods and Analytical Performance Characteristics of Immunological Assays for Human Immunoglobulin E (IgE) Antibodies of Defined Allergen Specificities; Approved Guideline (Clinical and Laboratory Standards Institute, document I/LA20-A)
  • Quality Assurance of Laboratory Tests for Autoantibodies to Nuclear Antigens: (1) Indirect Fluorescence Assay for Microscopy and (2) Microtiter Enzyme Immunoassay Methods (Clinical and Laboratory Standards Institute, document ILA02-A2)

CAP Products CAP Products

Surveys/EXCEL Proficiency Testing

Immunology by Survey

  • ACL - Anti-Cardiolipin Antibody and Beta-2 Glycoprotein I
  • AHT - Anti-Histone Antibodies
  • ANA - Anti-Nuclear Antibody
  • APC - Antiparietal Cell Antibody
  • ASC - Anti-Sacchromyces cerevisiae Antibody (ASCA)
  • ASO - Anti-Streptolysin O
  • CH50 - Total Hemolytic Complement
  • CCP-Cyclic Citrullinated Peptide Antibody (Anti-CCP)
  • CES - Celiac Serology
  • CRP - C-Reactive Protein
  • CTKN - Cytokines
  • G - Syphilis Serology
  • H - Hepatic Disease Serologies (antimitochondrial M2 antibody)
  • HCG - Human Chorionic Gonadotropin
  • IG - Immunology (General)
  • IM - Infectious Mononucleosis
  • IMW – Infectious Mononucleosis, waived
  • LKM - Liver Kidney Microsomal Antibody (Anti-LKM)
  • QF – M. tuberculosis detection using the QuantiFERON® TB method
  • RDS - Rheumatic Disease Serologies (Anti-Jo-1, Anti-Scl-70)
  • RF - Rheumatoid Factor
  • RUB - Rubella
  • S2 - Immunology (Special)
  • S4 - Immunology (Special, Limited)
  • S5 - Helicobactor pylori
  • SE - Diagnostic Allergy
  • SFLC - Serum Free Light Chains
  • V - Viscosity

Flow cytometry

  • FL (FL1- Lymphocyte Immunophenotyping, FL2 - DNA Content and Cell Cycle Analysis)
  • FL3 - Leukemia/Lymphoma
  • FL3CD Leukemia/Lymphoma CD-ROM
  • FL4 - CD34+
  • PNH - Analysis of RBCs and WBCs
  • FL5 - Flow Cytometry, Interpretation Only
  • ZAP70 – ZAP-70 Analysis by Flow Cytometry
  • RFAV1 and RFAV2 - Rare Flow Antigen Validation CD1a and CD103

Related Links Related Links