Originally published in CAP TODAY
The new checklist question will read, “Are positive and negative controls tested and results recorded for each new batch, lot number, and shipment of antisera when prepared or opened and once every six months thereafter?”
The requirement for performing quality control on Gram stains weekly has not changed.
Currently, non-immunofluorescent acid-fast stains must be checked each week of use. The new checklist edition will require that results be checked with positive controls each day of use.
The checklist currently requires fluorescent acid-fast stains to be controlled weekly or each day of use, whichever is less frequent. The new checklist will require that fluorescent stains be checked with positive and negative controls each time they are used.
In the current checklist, biochemical tests for mycobacterial identification must have positive controls run each day of use. The only mycobacterial biochemical test requiring a negative control is the iron uptake test. In the new checklist, the requirement for a negative control will be extended to all biochemical tests.