CAP ’13 – THE Pathologists’ Meeting – October 13 – 16, 2013™
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IWMD401  Profiling FFPE Samples with Next-Generation Sequencing, aCGH and FISH

Monday, October 14
6:15 PM

Deep Clonal Profiling of Formalin-Fixed, Paraffin-Embedded Clinical Samples
Michael T. Barrett, Ph.D.
Clinical Translational Research Division

Formalin-Fixed, Paraffin-Embedded (FFPE) tissues are a vast resource of annotated clinical tumor samples. However, their use in clinical research is limited by the variable quality of DNA extracted and admixtures of non-tumor and polyclonal neoplastic cell populations. To exploit archived FFPE tumor tissues, we developed DNA content based flow cytometry assays to sort the nuclei of clonal populations of neoplastic cells. Our methods yield highly purified (>95%) samples for analysis and provide templates suitable for aCGH and next generation sequencing (NGS) from a variety of FFPE tissues. These methods may be applied to advance research to enable more effective personalized medicine for cancer.

What’s New in HER2-Testing? Recommendations for HER2-Testing in Breast Cancer: ASCO/CAP Clinical Practice Guideline Update (2013)
David G. Hicks MD
Professor of Pathology and Lab Medicine
Director of the Surgical Pathology Unit
University of Rochester Medical Center
Rochester New York

Dr. David Hicks will provide an overview of the newly published ASCO/CAP guidelines and discuss implementation for breast cancer analysis. He will cover information about the new HER2 IQFISH pharmDx™ assay for breast and gastric HER2 testing.

Presented by Agilent





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