The CAP/American College of Medical Genetics and Genomics (ACMG) Cytogenetics Committee has developed criteria to evaluate cases for use in a CY Survey.
Use the following criteria when assessing a case for submission and provide all the information outlined. Cases can be submitted electronically.
If a case is chosen for use in a CY Survey, the submitting laboratory will be reimbursed $200.00 for each case.
- Select cases that are technically good examples of abnormalities a cytogenetics laboratory might reasonably expect to encounter.
- Complete the Clinical Information Form. The form requires information about the case history and laboratory contact person.
- Submit 8–10 cells with a minimum of 4 per cell line. For each cell, submit:
- Enhanced karyotype: Each karyotype must show that an overlap is present. Do not overtrim or mirror image chromosomes
- Unenhanced/raw metaphase
- Enhanced metaphase. Enhance images should be enhanced prior to chromosome cutting.
- Must be at least 96 dpi measuring 8 x 6 TIFF images. JPEG images cannot be submitted.
- No red boundary lines
- No fused box frames around chromosomes
- No halos around a chromosome (occurs when a chromosome is enhanced by itself, in karyotype mode)
- Fuse an entire cell into one frame for both sets of images. The enhanced and raw/unenhanced images need to match.
- Do not remove debris or nuclei that are close to a chromosome.
- Rename laboratory images with sequential numbers (eg, 1–10).
- Double check that all submitted cells:
- Are complete
- Are not in duplicate
- Are not flipped or rotated
- Have abnormal chromosomes separated and/or the region of the abnormality is not overlapped
- Every cell should be at the 400-band level or higher.
- Cells should have very few overlaps and, in particular, should not have overlaps in regions critical to characterizing
- Although it is preferred that cells with triple overlaps not be submitted, if this cannot be avoided, please draw attention to the overlap in your image (eg, draw a box around it).
- Results from other staining techniques can be provided when pertinent and of educational value.
- Cases submitted must be stripped of all patient identifiers, except for a unique laboratory identifier/laboratory name.
- Images should be submitted electronically to Jaimie Halley, CAP Surveys Department at email@example.com.